|
KMID : 0811720080120050245
|
|
Korean Journal of Physiology & Pharmacology
2008 Volume.12 No. 5 p.245 ~ p.252
|
|
|
Single-Channel Recording of TASK-3-like K+ Channel and Up- Regulation of TASK-3 mRNA Expression after Spinal Cord Injury in Rat Dorsal Root Ganglion Neurons
|
|
Jang In-seok
Lee Eun-Shin
Kang Da-Won
Han Jae-Hee
Kim Eun-Jin
Kim Guy-Tae
Ahn Sang-Ho
La Jun-Hon
Kee Jeong-Soon
Kiim So-Jeong
Seo Jeong-Min
Park Jae-Yong
Hong Seong-Geun
|
|
|
Abstract
|
|
|
|
Single-channel recordings of TASK-1 and TASK-3, members of two-pore domain K+ channel family, have not yet been reported in dorsal root ganglion (DRG) neurons, even though their mRNA and activity in whole-cell currents have been detected in these neurons. Here, we report single-channel kinetics of the TASK-3-like K+ channel in DRG neurons and up-regulation of TASK-3 mRNA expression in tissues isolated from animals with spinal cord injury (SCI). In DRG neurons, the single-channel conductance of TASK-3-like K+ channel was 33.0±0.1 pS at ?60 mV, and TASK-3 activity fell by 65±5% when the extracellular pH was changed from 7.3 to 6.3, indicating that the DRG K+ channel is similar to cloned TASK-3 channel. TASK-3 mRNA and protein levels in brain, spinal cord, and DRG were significantly higher in injured animals than in sham-operated ones. These results indicate that TASK-3 channels are expressed and functional in DRG neurons and the expression level is up-regulated following SCI, and suggest that TASK-3 channel could act as a potential background K＋ channel under SCI-induced acidic condition.
|
|
|
KEYWORD
|
|
|
Two-pore domain K+ channel, Dorsal root ganglion, Spinal cord injuries, Acidosis
|
|
|
FullTexts / Linksout information
|
|
 
|
|
|
Listed journal information
|
|
  
|